Measurement of Antigen-Specific IgG Titers by Direct ELISA
Bassel Akache1, Felicity C Stark1, Michael J McCluskie2
1Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2020
Abstract:
Direct ELISA allows for the measurement of antibody levels to a particular antigen. Serum or plasma from the vaccinated subject are incubated on high-binding capacity microplates precoated with the antigen of interest and detected utilizing an enzyme-linked secondary antibody. Herein, using influenza hemagglutinin as model antigen, we describe the quantification of antigen-specific IgG titers in mouse serum to measure vaccine-induced humoral responses.


