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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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Automated pH and conductivity conditioning using feedback control to support a two-step continuous purification

Ryan Jackobek¹, Sarah Herrick-Wagman², Lily Zhu²

¹BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Andover, MA 01810, United States.

Journal of Chromatography. A

|September 22, 2020

Summary

This summary is machine-generated.

Automated continuous chromatography systems enhance biopharmaceutical purification by increasing throughput and reducing costs. This novel instrument integrates multiple chromatography steps for efficient, hands-free operation in drug discovery and production.

Keywords:

Anion exchange Continuous Discovery Protein A ÄKTA pure 150 ÄKTA pcc

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Last Updated: Dec 8, 2025

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Area of Science:

Biopharmaceutical Manufacturing
Chromatography
Process Automation

Background:

Discovery research organizations require increased purification bandwidth for advancing molecules and modalities.
Automation and continuous processing technologies offer reduced manufacturing footprint and lower costs.
Early implementation of these technologies can accelerate development and production timelines.

Purpose of the Study:

To describe an automated continuous instrument for biopharmaceutical purification.
To enable efficient, hands-free operation for both development and production scales.
To facilitate screening of anion exchange chromatography conditions.

Main Methods:

An automated continuous system was designed, integrating an ÄKTA™ pcc for Protein A capture.
A retrofitted ÄKTA pure 150 automatically conditioned Protein A eluate.
A second ÄKTA pure 150 performed flow-through anion exchange chromatography with automated eluate recirculation and titrant addition for pH and conductivity control.

Main Results:

The developed instrument operates continuously without user intervention.
Precise adjustments to pH and salt concentration were achieved through automated titrant addition.
The system allows for continuous production or development screening of anion exchange conditions.

Conclusions:

The automated continuous chromatography instrument successfully integrates multiple purification steps.
This system enhances efficiency, reduces manual intervention, and supports accelerated biopharmaceutical development.
The technology provides a valuable tool for optimizing purification processes in drug discovery and manufacturing.