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GuttaFlow® Bioseal Cytotoxicity Assessment: In Vitro Study
Inês Ferreira1,2, Mafalda Laranjo2,3,4,5, Carlos Miguel Marto3,4,5,6
1Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal.
Molecules (Basel, Switzerland)
|September 23, 2020
Summary
GuttaFlow® bioseal exhibits superior biocompatibility compared to AH26® epoxy resin in root canal sealers. GuttaFlow® bioseal maintained cell viability and metabolic activity, while AH26® severely compromised cellular functions.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Cell Biology
Background:
- Root canal sealers are critical for successful endodontic treatment.
- Biocompatibility of sealers with peri-radicular tissues is essential for long-term outcomes.
- Epoxy resin-based sealers like AH26® are commonly used, but their cytotoxicity is a concern.
Purpose of the Study:
- To evaluate the cytotoxic effects of GuttaFlow® bioseal sealer.
- To compare the biocompatibility of GuttaFlow® bioseal with AH26® epoxy resin.
- To assess the impact of these sealers on odontoblast cell viability, metabolism, and mineralization.
Main Methods:
- Conditioned media from GuttaFlow® bioseal and AH26® were used to treat MDPC-23 odontoblast cell cultures.
- MTT and SRB assays evaluated metabolic activity and cellular viability.
- Reactive oxygen species production, cell cycle, cell death, and mineralization capacity were assessed using DHE, DCF-DA, cytometry, and Alizarin Red S staining, respectively.
Main Results:
- GuttaFlow® bioseal maintained high metabolic activity and cell viability, with minor effects at high concentrations.
- AH26® significantly compromised metabolic activity and induced cell death, including apoptosis and necrosis.
- GuttaFlow® bioseal demonstrated a better capacity to maintain cell functions and mineralization compared to AH26®.
Conclusions:
- GuttaFlow® bioseal exhibits significantly higher biocompatibility than AH26® epoxy resin.
- GuttaFlow® bioseal is a promising alternative for root canal treatment due to its favorable cellular response.
- Further in vivo studies are warranted to confirm the clinical efficacy and safety of GuttaFlow® bioseal.
