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Tunicamycin induced endoplasmic reticulum stress in the small intestine

Zübeyde Öztel1, Sibel Gazan1, Erdal Balcan1

  • 1Department of Biology, Manisa Celal Bayar University, Manisa, Turkey.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|September 23, 2020
PubMed

Insights

Short-term endoplasmic reticulum (ER) stress from tunicamycin (TN) did not impact leukocyte migration in the small intestine. However, it significantly decreased PECAM-1 expression in villi, altering intestinal villus architecture.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • The small intestine's immune response is crucial due to constant exposure to foreign substances.
  • Endoplasmic reticulum (ER) stress is implicated in various cellular processes and disease states.

Purpose of the Study:

  • To investigate the effect of tunicamycin (TN)-induced ER stress on the expression of MAdCAM-1, PECAM-1 (CD31), and CAV-1 in the small intestine.
  • To determine if ER stress impacts key molecules involved in immune cell trafficking and intestinal structure.

Main Methods:

  • Mice were administered a single intraperitoneal dose of tunicamycin (TN).
  • Expression levels of MAdCAM-1, PECAM-1, and CAV-1 were analyzed in Peyer's patches and villi 24 hours post-treatment.
  • Immunohistochemistry (IHC), immunofluorescence (IF), and western blotting were employed for analysis.

Main Results:

  • MAdCAM-1 and CAV-1 expression patterns remained similar in both control and TN-treated groups.
  • PECAM-1 immunoreactivity showed no significant change in Peyer's patches.
  • A significant decrease in PECAM-1 staining was observed in the villi of TN-treated mice.

Conclusions:

  • Brief ER stress induced by tunicamycin does not impede leukocyte migration to the small intestine's lymphoid compartments.
  • Tunicamycin treatment significantly reduces PECAM-1 expression in intestinal villi, leading to alterations in villus architecture.
Keywords:
CAV-1MADCAM-1PECAM-1Peyer’s patchesendotheliumleukocyte migrationmousereticulumstresstunicamycin

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