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Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Related Experiment Video

Updated: Dec 8, 2025

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Bioluminescent Imaging Systems for Assay Developments.

Sung-Bae Kim¹, Ramasamy Paulmurugan²

¹Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, 305-8569, Japan. kimu-sb@aist.go.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|September 23, 2020

Summary

This summary is machine-generated.

Bioluminescence (BL) offers a powerful optical readout for bioassays and imaging due to its high signal and simplicity. This review guides researchers through diverse BL systems, addressing challenges in selection and standardization for effective assay design.

Keywords:

BRET Bioluminescence (BL)assay coelenterazine imaging luciferase luciferin probe protein-fragment complementation assay (PCA)

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Area of Science:

Biochemistry
Molecular Biology
Bioanalytical Chemistry

Background:

Bioluminescence (BL) is a highly sensitive optical readout with advantages like a wide dynamic range, high signal-to-noise ratio, and no need for an external excitation source.
BL is versatile for cell-based assays, molecular imaging, and various bioassays due to its biocompatibility and simple measurement systems.
Recent research has expanded the library of luciferase-luciferin systems, offering diverse tools for bioassays.

Purpose of the Study:

To provide an overview of recent developments and applications in bioluminescence studies.
To showcase the bioanalytical utilities of various bioluminescence systems.
To offer guidance for selecting appropriate bioluminescence tools for specific assay designs.

Main Methods:

Literature review of recent advancements in bioluminescence.
Analysis of diverse luciferase-luciferin systems and their applications.
Discussion of challenges and standardization in bioluminescence assay development.

Main Results:

Bioluminescence offers significant advantages for bioassays and molecular imaging.
The diversity of BL systems presents challenges in selection and standardization.
A comprehensive overview of BL technologies and their bioanalytical applications is presented.

Conclusions:

Bioluminescence is a valuable tool in bioassays and molecular imaging.
Standardization and clear guidance are needed to effectively utilize the growing diversity of BL systems.
This review serves as a reference for researchers designing bioluminescence-based assays.