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Updated: Dec 8, 2025
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
Published on: July 4, 2018
Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats
Hery Poerwosusanta1, Gunadi2, Ika Kustiyah Oktaviyanti3
1Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia.
Background:
Laparoscopic procedures under certain pressure have the potential to cause intra-abdominal adhesions. However, the pathomechanism of this disorder is unknown. Release of mast cell mediators due to mast cell degranulation is thought to be the cause.
Materials And Methods:
Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per group): one control group and four intervention groups to which 60 min insufflation was performed using carbon dioxide at 5, 8, 10 and 12 mmHg. Seven days after laparoscopy, we euthanized and evaluated the levels of histamine, tryptase, and chymase of peritoneal fluid, the thickness of ECM of peritoneal tissue, and intraabdominal adhesion scoring system.
Results:
Histamine and tryptase levels in peritoneal fluid were significantly higher at the 10- and 12 mm Hg intervention compared to control (histamine: 0.50 ± 0.35 vs. 0.41 ± 0.41 vs. 0.04 ± 0.02 ng/mL, respectively; and tryptase: 0.69 ± 0.11 vs. 0.65 ± 0.05 vs. 0.48 ± 0.02 ng/ml respectively). The ECM was significantly thicker in the intervention groups at 10- and 12-mm Hg compared to control (71.3 [66.7-85.2] vs. 48.4 [34.5-50.3] vs. 10.25 [8.7-12.1] μm, respectively). Moreover, the intra-abdominal scoring was also significantly higher in the intervention groups at 10- and 12 mm Hg compared to control (4 [0-4] vs. 4.5 [4-5], vs. 0, respectively).
Conclusions:
Laparoscopic procedures increase the release of mast cell mediators in peritoneal fluid, the thickness of ECM and intraabdominal adhesion scoring in rats, implying that it might increase the possibility of intrabdominal adhesion in humans.
Insights
Laparoscopic procedures increase mast cell mediator release and extracellular matrix thickness, potentially raising the risk of intra-abdominal adhesions in humans. This study investigated the link between insufflation pressure and adhesion development in rats.
Area of Science:
- Surgical Innovation
- Gastroenterology
- Pathophysiology
Background:
- Laparoscopic procedures, particularly under elevated intra-abdominal pressure, are associated with intra-abdominal adhesions.
- The precise mechanism leading to these adhesions remains unclear, but mast cell degranulation and mediator release are suspected.
- Understanding this pathway is crucial for preventing post-surgical complications.
Purpose of the Study:
- To investigate the relationship between carbon dioxide insufflation pressure during laparoscopy and the development of intra-abdominal adhesions in a rat model.
- To evaluate the impact of varying insufflation pressures on mast cell mediator levels, extracellular matrix (ECM) thickness, and adhesion scoring.
Main Methods:
- Thirty male Sprague-Dawley rats were divided into a control group and four intervention groups.
- Intervention groups underwent 60-minute carbon dioxide insufflation at pressures of 5, 8, 10, and 12 mmHg.
- Peritoneal fluid and tissue were analyzed for histamine, tryptase, chymase, ECM thickness, and intra-abdominal adhesion scores seven days post-procedure.
Main Results:
- Significantly elevated levels of histamine and tryptase were observed in the 10 and 12 mmHg groups compared to controls.
- Extracellular matrix thickness and intra-abdominal adhesion scores were significantly higher in the 10 and 12 mmHg groups.
- These findings indicate a pressure-dependent increase in inflammatory mediators and adhesion formation.
Conclusions:
- Laparoscopic insufflation pressure is linked to increased mast cell mediator release and extracellular matrix deposition in rats.
- The study suggests a potential mechanism by which laparoscopic procedures may increase the risk of intra-abdominal adhesions in humans.
- Further research is warranted to confirm these findings and explore preventative strategies.
