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Updated: Dec 8, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated
Zhipeng Huang1, Qiuyuan Lin1, Bin Yang1
1Department of Chemistry, Fudan University, Shanghai 200438, China. chenhui@fudan.edu.cn wengwenhao2010@163.com jlkong@fudan.edu.cn.
Abstract:
A novel cascade signal amplification based on tyramide signal amplification (TSA) and surface-initiated enzymatic polymerization (SIEP) was first reported for the sensitive and template-free detection of colorectal cancer (CRC) exosomes. This assay exhibited 20.9-fold signal amplification with a low detection limit of 12.8 particles per μL. Furthermore, accurate and reproducible results were obtained for detecting exosomes in serum samples, suggesting its potential application in exosomes analysis and clinical diagnostics.

