Photocurable platelet rich plasma bioadhesives

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing Research

Background:

• Current synthetic tissue adhesives present toxicity and workability issues.
• Blood-derived adhesives offer resorption but lack sufficient adhesion for reliable wound closure.
• A need exists for advanced wound dressings combining effective adhesion and healing properties.

Purpose of the Study:

• To develop a semi-synthetic bioadhesive composite with enhanced properties for wound closure.
• To combine the benefits of synthetic and blood-derived adhesives into a single formulation.
• To evaluate the efficacy of the novel bioadhesive in promoting wound healing and reducing inflammation.

Main Methods:

• Formulation of a novel semi-synthetic bioadhesive: PAMAM-g-diazirine (PDz) blended with platelet-rich plasma (PRP) to create the PDz/PRP composite.
• Characterization of the PDz/PRP composite for properties such as gelling time, miscibility, elasticity, and light activation.
• In vivo assessment of the PDz/PRP composite on full-thickness wounds to evaluate its hemostatic properties and impact on inflammation.

Main Results:

• The PDz/PRP composite exhibits rapid gelling and is miscible in aqueous and organic solvents.
• The light-activated PDz/PRP composite demonstrates elasticity similar to soft tissues and functions as an induced hemostat.
• Application of the PDz/PRP composite to in vivo full-thickness wounds resulted in a 25% reduction in inflammation.

Conclusions:

• The developed PDz/PRP composite represents a promising semi-synthetic bioadhesive for wound dressing applications.
• This novel formulation effectively combines strong adhesion with potent wound healing capabilities.
• The observed reduction in inflammation highlights the potential of PDz/PRP as an advanced wound healing technology.