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Application of "Tissueoid Cell Culture System" Using a Silicate Fiber Scaffold for Cancer Research.

Shoko Murakami1,2, Ken-Ichi Mukaisho3, Takuya Iwasa4

  • 1Division of Molecular and Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Otsu, Japan.

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
|September 23, 2020
PubMed
Summary

A novel 3D culture system, Cellbed™, using silica fiber scaffolds, successfully mimics cancer cell morphology in vivo. Coating with collagen IV enhances cancer cell invasion and proliferation, offering a better understanding of tumor microenvironments.

Keywords:
Cancer researchCell cultureSilica fiberThree-dimensional culture systemTissueoid

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Area of Science:

  • Biotechnology
  • Cancer Research
  • Cell Biology

Background:

  • A novel 3-dimensional (3D) culture system, Cellbed™, was developed using unwoven sheets of high-purity silica fiber scaffolds.
  • This system aims to better recapitulate the in vivo microenvironment for cancer cell studies.

Purpose of the Study:

  • To evaluate the utility of the Cellbed™ 3D culture system for various cancer cell types.
  • To investigate the effects of extracellular matrix components on cancer cell behavior within the 3D system.

Main Methods:

  • Adherent (colon, esophagogastric junction adenocarcinoma, tongue squamous cell carcinoma - SqCC) and nonadherent (gastric cancer) cell lines were cultured.
  • Cells were analyzed using electron microscopy (scanning and transmission).
  • SqCC cells were cultured on Cellbed™ scaffolds coated with collagens I, III, and IV to assess extracellular matrix effects.

Main Results:

  • Well-differentiated adenocarcinoma cells formed lumens, and SqCC cells exhibited stratification and keratinization in the 3D culture.
  • Electron microscopy confirmed cancer cell proliferation and the presence of intercellular desmosomes.
  • Collagen IV coating significantly enhanced SqCC cell invasion and proliferation.

Conclusions:

  • The developed 3D system, termed "tissueoid cell culture system," effectively mimics in vivo cancer cell morphology.
  • Collagen IV coating allows for modulation of cell-matrix interactions and better recapitulation of the in vivo tumor microenvironment.