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A proper placental sampling for syncytin-1 analysis.

Petra Priščáková1, Miroslav Korbeľ2, Zuzana Nižňanská2

  • 1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic.

Biotechniques
|September 24, 2020
PubMed
Summary

Syncytin-1 (ERVW-1) expression varies greatly in placental tissue. Analyzing multiple individual biopsies, not pooled samples, is crucial for accurate pre-eclampsia research, with random sampling recommended.

Keywords:
ERVW-1gene expression analysisplacentaplacental samplingpre-eclampsiasyncytin-1

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Area of Science:

  • Reproductive Biology
  • Genetics
  • Obstetrics

Background:

  • Syncytin-1 (ERVW-1) is investigated as a potential biomarker for pre-eclampsia and placental development issues.
  • Placental tissue heterogeneity necessitates standardized sampling methods for reliable analysis.

Purpose of the Study:

  • To assess the variability of ERVW-1 expression in placental biopsies.
  • To determine the optimal method for placental tissue sampling for gene expression analysis.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) was used to measure ERVW-1 expression levels.
  • Multiple biopsies (3-30) were taken from 44 placentae.
  • Individual analysis of ten biopsies per placenta was performed.

Main Results:

  • Significant variability in ERVW-1 expression was observed across different biopsies from the same placenta.
  • No correlation was found between biopsy location and ERVW-1 expression levels.
  • Individual analysis of multiple biopsies is recommended over pooled samples.

Conclusions:

  • High intra-placental variability of ERVW-1 expression necessitates individual analysis of multiple biopsies.
  • Random placental sampling, such as a long cut from the umbilical cord to the edge, is recommended for consistent results.
  • Accurate assessment of Syncytin-1 expression requires careful consideration of tissue sampling strategies.