Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.3K
Ribosome Profiling02:24

Ribosome Profiling

3.9K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dissecting Agronomically favorable genotypes in temperate japonica rice via haplotype analysis of a Japan-MAGIC population.

The Plant journal : for cell and molecular biology·2026
Same author

Differential regulation of heterochromatin and euchromatin by GmDDM1 during seed development ensures seedling viability in soybean.

The Plant journal : for cell and molecular biology·2026
Same author

Biomarker genes for model-based prediction of drought-stress perception levels in rice.

BMC plant biology·2025
Same author

Evaluation of transgenic rice seeds expressing T cell epitopes of Japanese cedar pollen allergens.

Transgenic research·2025
Same author

Effect of heterozygous deletions on phenotypic changes and dosage compensation in Arabidopsis thaliana.

Scientific reports·2025
Same author

Total RNA Extraction from Rice Vegetative Tissues Using Magnetic Beads.

Methods in molecular biology (Clifton, N.J.)·2024

Related Experiment Video

Updated: Dec 8, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.6K

Low-cost RNA extraction method for highly scalable transcriptome studies.

Kanami Yoshino1, Ryo Nishijima1, Taiji Kawakatsu1

  • 1Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 3-1-3 Kan-nondai, Tsukuba, Ibaraki 305-8604, Japan.

Breeding Science
|September 24, 2020
PubMed
Summary

A new, cost-effective RNA extraction method, HighGI, offers high-throughput preparation for large-scale transcriptome studies. This phenol-free protocol yields high-quality RNA, including small RNA fractions often lost in commercial kits.

Keywords:
guanidinium thiocyanatehigh-throughput RNA extractionmagnetic beadssmall RNA extraction

More Related Videos

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

183
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.8K

Related Experiment Videos

Last Updated: Dec 8, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.6K
Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

183
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.8K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • RNA extraction is crucial for transcriptome studies.
  • Existing methods face limitations in high-throughput applications and small RNA recovery.
  • Need for efficient, cost-effective RNA preparation protocols.

Purpose of the Study:

  • To develop a high-throughput RNA extraction method for large-scale transcriptome studies.
  • To establish a cost-effective and phenol-free RNA preparation protocol.
  • To evaluate the efficiency and yield of small RNA recovery.

Main Methods:

  • Development of a high-throughput guanidinium thiocyanate and isopropyl alcohol-based RNA extraction method (HighGI).
  • Utilized simple, homemade, phenol-free buffers.
  • Compared HighGI with conventional phenol/chloroform and commercial column-based kits.

Main Results:

  • HighGI demonstrated comparable RNA quality and quantity to conventional methods.
  • The method successfully retained small RNAs (<200 bp), unlike commercial kits.
  • HighGI is suitable for semi-automated RNA extraction, enabling high-throughput processing.

Conclusions:

  • HighGI provides a high-yield, high-quality RNA extraction solution for large-scale transcriptome studies.
  • The method is cost-effective and superior in small RNA recovery.
  • HighGI is readily adaptable for semi-automated, high-throughput RNA preparation.