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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
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Related Experiment Video

Updated: Dec 8, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Acoustic Mist Ionization-Mass Spectrometry: A Comparison to Conventional High-Throughput Screening and Compound

Arseniy M Belov, Joseph Kozole, Mark F Bean

  • 1Beckman Coulter Life Sciences, 170 Rose Orchard Way, San Jose, California 95134, United States.

Analytical Chemistry
|September 24, 2020
PubMed
Summary

Acoustically-mediated mass spectrometry (AMI-MS) offers a faster, cheaper, and more efficient high-throughput screening (HTS) method for drug discovery. This label-free technology accurately identifies drug candidates by measuring enzyme inhibition, reducing costs and improving hit rates compared to traditional assays.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Drug Discovery

Background:

  • Conventional high-throughput screening (HTS) methods are often costly, complex, and prone to interference, leading to high attrition rates.
  • Radioactivity-based assays and standard mass spectrometry (MS) platforms present limitations in cost, disposal, speed, and sample preparation for large-scale screening.

Purpose of the Study:

  • To develop and validate a high-throughput screening (HTS)-compatible assay using an acoustically-mediated mass spectrometry (AMI-MS) platform.
  • To assess the efficiency, cost-effectiveness, and accuracy of AMI-MS for measuring enzyme inhibition compared to conventional methods.

Main Methods:

  • Development of an HTS assay on the AMI-MS platform to measure histone acetyltransferase (HAT) activity inhibition.
  • Real-time kinetic measurements to determine enzyme kinetic parameters (Km and Vmax).
  • Comparative analysis of AMI-MS performance against Scintillation Proximity Assay (SPA) HTS and RapidFire-MS for hit identification and IC50 determination.

Main Results:

  • The AMI-MS assay successfully identified 86% of validated hits (pIC50 ≥ 5.0) from a previous SPA HTS campaign.
  • AMI-MS demonstrated a lower hit rate and significantly reduced cost per well compared to the SPA-based readout.
  • pIC50 values obtained via AMI-MS showed good correlation with those measured by RapidFire-MS.

Conclusions:

  • AMI-MS provides a highly selective, label-free, and efficient platform for high-throughput bioassays.
  • This technology offers significant improvements over conventional HTS methods, including reduced costs and enhanced accuracy in hit identification.
  • AMI-MS has the potential to revolutionize early-stage drug discovery by enabling faster and more economical screening of large compound libraries.