Development of fast multi-slice apparent T₁ mapping for improved arterial spin labeling MRI measurement of cerebral...

To develop fast multi-slice apparent T₁ (T_1app ) mapping for accurate cerebral blood flow (CBF) quantification with arterial spin labeling (ASL) MRI.

Fast multi-slice T_1app was measured using a modified inversion recovery echo planar imaging (EPI) sequence with simultaneous application of ASL tagging radiofrequency (RF) and gradient pulses. The fast multi-slice T_1app measurement was compared with the single-slice T_1app imaging approach, repeated per slice. CBF was assessed in healthy adult Wistar rats (N = 5) and rats with acute stroke 24 hours after a transient middle cerebral artery occlusion (N = 5).

The fast multi-slice T_1app measurement was in good agreement with that of a single-slice T_1app imaging approach (Lin's concordance correlation coefficient = 0.92). CBF calculated using T_1app reasonably accounted for the finite labeling RF duration, whereas the routine T₁ -normalized ASL MRI underestimated the CBF, particularly at short labeling durations. In acute stroke rats, the labeling time and the CBF difference (ΔCBF) between the contralateral normal area and the ischemic lesion were significantly correlated when using T₁ -normalized perfusion calculation (R = 0.844, P = .035). In comparison, T_1app -normalized ΔCBF had little labeling time dependence based on the linear regression equation of ΔCBF = -0.0247*τ + 1.579 mL/g/min (R = -0.352, P = .494).

Our study found fast multi-slice T_1app imaging improves the accuracy and reproducibility of CBF measurement.