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Related Experiment Video

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Single-Cell Elasticity Measurement with an Optically Actuated Microrobot.

István Grexa1,2, Tamás Fekete1,3, Judit Molnár1

  • 1Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary.

Micromachines
|September 25, 2020
PubMed
Summary

This study introduces a novel method for measuring cell elasticity using polymer microtools and holographic optical tweezers. This technique offers a precise and gentle approach for probing cell mechanical properties.

Keywords:
cell elasticityendothelial cellsholographic optical tweezersimage processingoptical micromanipulationtwo-photon polymerization

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Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Accurate measurement of cell elasticity is crucial for understanding cellular mechanics and disease.
  • Existing methods for cell elasticity measurement have limitations in force range and potential for cell photodamage.

Purpose of the Study:

  • To develop and validate a new method for measuring cell elasticity using polymer microtools actuated by holographic optical tweezers.
  • To demonstrate the feasibility of this technique for probing endothelial cells.

Main Methods:

  • Fabrication of polymer microtools using two-photon polymerization.
  • Actuation of microtools using holographic optical tweezers for precise manipulation.
  • Lateral probing of endothelial cells and analysis of tool position via video microscopy and image processing.
  • Measurement of forces down to the femtonewton (fN) regime.

Main Results:

  • The developed method allows for cell elasticity measurements with high precision.
  • The specially shaped microtools minimize photodamage to the cells during optical trapping.
  • Young's modulus values obtained were critically compared with existing literature data.
  • The technique successfully probed endothelial cells in a lateral direction.

Conclusions:

  • This approach provides a feasible and sensitive alternative to conventional vertical indentation experiments for cell elasticity measurements.
  • Holographic optical tweezers enable an extended force range for cell indentation studies.
  • The method holds promise for advancing the field of cell mechanics research.