Age- and Sex-Related Modifications of Hematology in Spanish Purebred Horse

The purpose of this study was to test the hypothesis that age and sex significantly modulate the changes in hematological parameters of physiologically healthy Spanish Purebred horse, establishing the reference values. Blood samples were obtained on a total of 159 horses (n = 77 males and n = 82 females), classified in four age groups: group A (1-2 years; n = 39), group B (>2-3 years; n = 38), group C (>3-4 years; n = 41), and group D (>4-7 years; n = 41). By means ADVIA 2020i, red blood cell (RBC), hemoglobin concentration (HB), packed cell volume, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration, platelets (PLTs), as well as total and differential white blood cells (WBCs) were determined. Total plasma protein was determined by spectrophotometry. Results obtained showed that sex had a limited influence on these parameters, being the HB higher in males and WBC higher in females (P = .047). Age caused a reduction in RBC, with a compensatory increase of MCV and MCH, decrease in WBC, PLT, lymphocytes (LYMPs), as well as an increase of N/L ratio (P = .047). Interactions between age and sex showed higher RBC in males than in females of the group B, and higher HB and WBC in males than in females of the group D. According to hematological data, Spanish Purebred horse was classified as a warm- or cold-blooded horse. Decline of RBC, WBC, and PLT parameters is a natural condition of horse with the advance of age, indicating a decrease of the narrow bone response.