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pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110
Lena Schaffert1, Lucas Jacob1, Susanne Schneiker-Bekel1,2
1Microbial Genomics and Biotechnology, Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
Microbiology Resource Announcements
|September 25, 2020
Abstract:
The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.
