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Related Concept Videos

Structural Isomerism02:34

Structural Isomerism

Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can be...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

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Related Experiment Video

Updated: May 26, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Multiple exon-binding sites in class II self-splicing introns.

A Jacquier, F Michel

    Cell
    |July 3, 1987
    PubMed
    Summary

    Investigating a yeast mitochondrial intron revealed specific intron-exon binding sites crucial for self-splicing. Restoring these interactions in mutants normalized the splicing process, highlighting their importance in RNA splicing.

    Area of Science:

    • Molecular Biology
    • RNA Biology
    • Yeast Genetics

    Background:

    • Class II introns are self-splicing RNAs found in various organisms, including yeast mitochondria.
    • Intron-exon interactions are critical for the precise splicing of precursor RNA molecules.
    • Understanding these interactions is key to deciphering the mechanisms of RNA splicing.

    Purpose of the Study:

    • To identify and characterize specific sites of interaction between S. cerevisiae mitochondrial intron a5 and its 5' exon.
    • To investigate the functional significance of these intron-exon binding sites in the splicing process.
    • To explore the role of these interactions in the catalytic steps of splicing.

    Main Methods:

    • Partial deletion analysis of the 5' exon to reveal intron-exon interaction sites.

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    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
    06:28

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

    Published on: September 2, 2025

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    Last Updated: May 26, 2026

    Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
    07:08

    Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

    Published on: July 14, 2015

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
    10:59

    Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
    06:28

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

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  • Comparative sequence analysis to identify potential binding regions within the intron.
  • RNAase H digestion assays using a DNA version of the 5' exon to map binding sites.
  • Site-directed mutagenesis of intronic and exonic sequences to assess functional impact on splicing in vitro.
  • Analysis of splicing intermediates, including the intron-3' exon lariat.

    • Main Results:

    • Several sites of intron-exon interaction were identified in S. cerevisiae intron a5.
    • Two specific exon-binding sites within intron a5 were mapped using comparative sequence analysis and RNAase H digestion.
    • Mutations in these intronic or exonic sites impaired in vitro splicing, while restoring the pairings rescued splicing activity.
    • Accumulation of the intron-3' exon lariat intermediate was observed in certain mutants, supporting its role in splicing.

    Conclusions:

    • Specific intron-exon interactions are essential for the efficient and accurate splicing of yeast mitochondrial class II introns.
    • The identified intronic sites play a crucial role in mediating these interactions, potentially by aligning exons for ligation.
    • These findings provide insights into the molecular mechanisms governing RNA splicing and the role of RNA-RNA interactions.