Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.7K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distributed Jamming Method for ASLC Systems Based on Random Phase Perturbation.

Sensors (Basel, Switzerland)·2026
Same author

Coupling conversion of CO/CO<sub>2</sub> to chemicals through zeolite catalysis.

Chemical science·2026
Same author

Personalized therapeutic platform: organoid models for hepatic malignancies.

Journal of translational medicine·2026
Same author

Extra-large pore JZO zeolites with tunable Si/Al ratios as efficient catalysts for degradable plastic monomer production.

RSC advances·2026
Same author

Spatiotemporally programmed nanomedicine engineering to resolve conflicting immunosignals in triple-negative breast cancer.

Signal transduction and targeted therapy·2026
Same author

<i>Pythium</i> <i>oligandrum</i> Is a Type of Biocontrol Oomycete with Great Potential.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Engineering <i>Lactococcus cremoris</i> strains co-expressing two cellulase genes for growth on cellulose.

Frontiers in bioengineering and biotechnology·2026
Same journal

Exosome-mediated tendon-derived stem cell therapy strategies: potential and challenges.

Frontiers in bioengineering and biotechnology·2026
Same journal

Evaluating the effects of glutaraldehyde concentration and incubation time on the structural integrity of the human pericardium.

Frontiers in bioengineering and biotechnology·2026
Same journal

Effect of hypergravity on the biomechanics of the musculoskeletal system in human lumbar spine: a numerical study.

Frontiers in bioengineering and biotechnology·2026
Same journal

Immunomodulatory and cytotoxic effects of dental methacrylates: a narrative review focusing on 2-hydroxyethyl methacrylate and triethylene glycol dimethacrylate.

Frontiers in bioengineering and biotechnology·2026
Same journal

Optimizing biogas production from poultry manure and cocoa pod husks co-digestion: implications for circular bioeconomy and decentralized energy systems in West Africa.

Frontiers in bioengineering and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Dec 7, 2025

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.6K

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis.

Jieqing Lei1, Liang Qi1, Zhigang Luo1,2,3

  • 1School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Frontiers in Bioengineering and Biotechnology
|September 25, 2020
PubMed
Summary
This summary is machine-generated.

We developed robust metal-organic framework (MOF) microcapsules for enzyme immobilization. These novel microcapsules offer excellent catalytic activity, stability, and size selectivity for biocatalysis, with easy separation via sedimentation.

Keywords:
ZIF-8biocatalysisenzyme immobilizationmicrocapsulesself-assemblysize selectivity

More Related Videos

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.3K
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.7K

Related Experiment Videos

Last Updated: Dec 7, 2025

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.6K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.3K
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.7K

Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • Enzyme immobilization is crucial for biocatalysis, enhancing stability and reusability.
  • Metal-organic frameworks (MOFs) offer tunable properties for advanced material design.
  • Developing efficient microcapsules for enzyme delivery remains a key challenge.

Purpose of the Study:

  • To develop a mild and effective method for preparing MOF-based microcapsules for enzyme immobilization.
  • To achieve controlled enzyme loading within the microcapsule structure (shell or cavity).
  • To evaluate the catalytic performance, stability, and selectivity of the enzyme-loaded microcapsules.

Main Methods:

  • Fabrication of zeolite imidazolate framework-8 (ZIF-8) microcapsules via Pickering emulsion and interfacial self-assembly.
  • Enzyme immobilization by incorporating Candida antarctica lipase B (CalB) into the ZIF-8 stabilizer or the aqueous phase.
  • Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and fluorescence microscopy.
  • Assessment of catalytic activity and selectivity in transesterification reactions with substrates of varying sizes.

Main Results:

  • Successfully synthesized robust core-shell ZIF-8 microcapsules with encapsulated CalB (CalB@ZIF-8@cap or ZIF-8@cap-CalB).
  • Confirmed enzyme encapsulation and location within the microcapsules via spectroscopic and microscopic techniques.
  • Demonstrated high catalytic activity, stability, and size selectivity in transesterification reactions.
  • Achieved efficient catalyst separation through sedimentation due to the microcapsule size.

Conclusions:

  • The developed MOF microcapsules provide a versatile platform for enzyme immobilization.
  • The microcapsules exhibit excellent catalytic performance and enable facile separation, reducing energy consumption.
  • These multifunctional MOF-enzyme composites hold promise for advanced biocatalysis and microreactor applications.