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Emergence of plasmid-borne bla oxa-181 gene in Ochrobactrum intermedium: first report from India

Thamaraiselvan Shanthini1, Prasanth Manohar1, Sagadevan Samna1

  • 1Antibiotic Resistance and Phage Therapy Laboratory, Department of Biomedical Sciences, School of Bioscience and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.

Access Microbiology
|September 25, 2020
PubMed

Insights

Wastewater harbors carbapenem-resistant bacteria. This study identified Ochrobactrum intermedium, a rare pathogen, carrying the OXA-181 gene in sewage, highlighting a novel public health concern.

Area of Science:

  • Environmental microbiology
  • Clinical microbiology
  • Molecular biology

Background:

  • Wastewater serves as a reservoir for antibiotic-resistant bacteria.
  • Multi-drug resistance poses a significant global health threat.

Purpose of the Study:

  • To investigate the prevalence of carbapenem-resistant bacteria in sewage.
  • To identify specific resistance genes and bacterial species in wastewater samples.

Main Methods:

  • Isolation of carbapenem-resistant non-lactose-fermenting Gram-negative bacteria (CR-NF-GNB) from hospital and non-hospital sewage using MacConkey agar with meropenem.
  • Screening for key beta-lactamase resistance genes (blaNDM, blaOXA-48-like, blaIMP, blaVIM, blaKPC).

Main Results:

  • 100 CR-NF-GNB isolates were obtained from 19 sewage samples.
  • Ochrobactrum intermedium carrying the plasmid-borne blaOXA-48-like gene was identified.
  • This marks the first report of O. intermedium encoding the OXA-181 gene.

Conclusions:

  • Sewage is a potential source for the dissemination of carbapenem resistance.
  • Ochrobactrum intermedium with OXA-181 represents an emerging opportunistic pathogen in wastewater.
  • Monitoring wastewater is crucial for tracking antibiotic resistance genes.
Keywords:
Gram-negative bacteriaOXAOchrobactrum intermediumcarbapenem-resistantcarbapenemasemulti-drug resistance

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