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Updated: Dec 7, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray.

Laura Luque-Sastre¹, Kieran Jordan², Séamus Fanning^1,3

¹Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)

|September 25, 2020

Summary

High-throughput screening methods offer a comprehensive view of bacterial phenotypes, aiding genotype correlations. This study applies OmniLog phenotype microarray analysis to characterize Listeria species.

Keywords:

Antimicrobial sensitivity Biochemical characterization Nutrient utilization OmniLog Phenotype Phenotypic microarray

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Area of Science:

Bacteriology
Microbiology
Biochemistry

Background:

High-throughput screening is crucial for bacterial phenotypic analysis.
Simultaneous measurement of multiple phenotype responses enhances investigation output.
Comprehensive bacterial phenotype overviews facilitate genotype-phenotype correlations.

Purpose of the Study:

To describe the application of OmniLog phenotype microarray analysis.
To characterize bacterial strains across various traits.
To investigate Listeria species using high-throughput assays.

Main Methods:

OmniLog phenotype microarray analysis was employed.
High-throughput assay for phenotypic characterization.
Analysis covered nutrient utilization and antimicrobial sensitivity.

Main Results:

The study successfully applied OmniLog analysis to Listeria species.
Detailed phenotypic profiles were generated.
Characterization across diverse traits was achieved.

Conclusions:

OmniLog phenotype microarray analysis is effective for Listeria characterization.
This high-throughput method provides a comprehensive overview of bacterial phenotypes.
Facilitates large-scale genotype-phenotype correlation studies.