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Complementary DNA for human T-cell cyclophilin.

B Haendler, R Hofer-Warbinek, E Hofer

    The EMBO Journal
    |April 1, 1987
    PubMed
    Summary
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    Researchers isolated and sequenced complementary DNA for human cyclophilin, a cyclosporin A-binding protein, from T-cells. Human cyclophilin shows minor differences from bovine cyclophilin and may belong to a multigene family.

    Area of Science:

    • Molecular Biology
    • Immunology
    • Genetics

    Background:

    • Cyclophilin is a specific cyclosporin A-binding protein.
    • Understanding human cyclophilin is crucial for immunology and drug development.

    Purpose of the Study:

    • To isolate and sequence complementary DNA (cDNA) encoding human cyclophilin.
    • To compare human cyclophilin with its bovine counterpart.
    • To investigate the expression and gene family of human cyclophilin.

    Main Methods:

    • Complementary DNA isolation and sequencing from Jurkat T-cell line.
    • Amino acid sequence comparison between human and bovine cyclophilin.
    • RNA transfer blot analysis for mRNA size determination.
    • Southern blot analysis for gene family investigation.

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    Main Results:

    • Human cyclophilin cDNA was successfully isolated and sequenced from T-cells.
    • Human cyclophilin exhibits high similarity to bovine cyclophilin, with only three amino acid differences.
    • Cyclophilin mRNA levels are only marginally affected by T-cell induction and cyclosporin A treatment.
    • Southern blot analysis suggests cyclophilin is encoded by a multigene family in humans.

    Conclusions:

    • Human cyclophilin is structurally similar to bovine cyclophilin.
    • The expression of cyclophilin mRNA is relatively stable under specific induction conditions.
    • The human genome likely contains multiple genes for cyclophilin, indicating functional diversity.