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Updated: Dec 7, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
An overview of applications of CRISPR-Cas technologies in biomedical engineering
Saleh Jamehdor1,2,3, Kasra Arbabi Zaboli4, Sina Naserian5,6
1Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract:
Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one of the major genome editing systems and allows changing DNA levels of an organism. Among several CRISPR categories, the CRISPR-Cas9 system has shown a remarkable progression rate over its lifetime. Recently, other tools including CRISPR-Cas12 and CRISPR-Cas13 have been introduced. CRISPR-Cas9 system has played a key role in the industrial cell factory's production and improved our understanding of genome function. Additionally, this system has been used as one of the major genome editing systems for the diagnosis and treatment of several infectious and non-infectious diseases. In this review, we discuss CRISPR biology, its versatility, and its application in biomedical engineering.
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