Related Experiment Video
Updated: Dec 7, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
The guanine nucleotide exchange factor FLJ00068 activates Rac1 in adipocyte insulin signaling
Nobuyuki Takenaka1, Mika Nakao1, Kiko Hasegawa1
1Laboratory of Cell Biology, Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Japan.
Abstract:
Insulin stimulates glucose uptake via the translocation of the glucose transporter GLUT4 to the plasma membrane in adipocytes. Several lines of evidence suggest that the small GTPase Rac1 plays an important role in insulin-stimulated glucose uptake in skeletal muscle and adipocytes. The purpose of this study is to investigate the mechanisms whereby Rac1 is regulated in adipocyte insulin signaling. Here, we show that knockdown of the guanine nucleotide exchange factor FLJ00068 inhibits Rac1 activation and GLUT4 translocation by insulin and a constitutively activated form of the protein kinase Akt2. Furthermore, constitutively activated FLJ00068 induced Rac1 activation and Rac1-dependent GLUT4 translocation. Collectively, these results suggest the involvement of FLJ00068 downstream of Akt2 in insulin-stimulated glucose uptake signaling in adipocytes.
Related Concept Videos
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Intracellular Signaling Affects Focal Adhesions
Some...
cAMP-dependent Protein Kinase Pathways
GPCRs Regulate Adenylyl Cylase Activity
The Ras Gene
Ras is a...
Insulin: The Receptor and Signaling Pathways

