Related Experiment Video

Updated: Dec 7, 2025

Single Molecule Analysis of Laser Localized Psoralen Adducts
11:46

Single Molecule Analysis of Laser Localized Psoralen Adducts

Published on: April 20, 2017

6.9K

New horizons of DNA adductome for exploring environmental causes of cancer

Yukari Totsuka1, Masatoshi Watanabe2, Yingsong Lin3

  • 1Department of Cancer Model Development, National Cancer Center Research Institute, Tokyo, Japan.

Cancer Science
|September 26, 2020
PubMed

Insights

Chemicals cause cancer by forming DNA adducts, which are DNA damages. DNA adductome analysis offers a new way to detect these adducts and understand cancer causes.

Area of Science:

  • Environmental Science
  • Molecular Biology
  • Toxicology

Background:

  • Chemical carcinogenesis involves DNA adduct formation, a critical DNA damage.
  • DNA adducts are biomarkers for environmental exposure and cancer risk.
  • Accurate measurement of DNA adducts in biological samples remains challenging.

Purpose of the Study:

  • To review the principles and applications of DNA adductome analysis.
  • To highlight its contribution to understanding environmental causes of cancer.
  • To present an integrated approach for cancer etiology research.

Main Methods:

  • DNA adductome analysis using mass spectrometry to screen multiple DNA adducts.
  • Integration of DNA adductome data with mutational signatures from whole-genome/exome sequencing.
  • Leveraging advances in next-generation sequencing.

Main Results:

  • DNA adductome analysis enables simultaneous screening of multiple DNA adducts with structural information.
  • This method aids in elucidating environmental factors contributing to cancer.
  • An integrated approach combining adductome data and mutational signatures offers new insights into cancer etiology.

Conclusions:

  • DNA adductome analysis is a powerful tool for investigating the environmental origins of cancer.
  • Integrating adductome data with genomic information advances cancer etiology research.
  • This approach holds promise for identifying cancer risks and developing preventive strategies.
Keywords:
DNA adductcancerenvironmental carcinogenmass spectrometrynext-generation sequencing

More Related Videos

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.2K
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.0K

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.7K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
40.1K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.8K
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.1K
Overview of DNA Repair02:25

Overview of DNA Repair

9.3K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
96.7K