Related Experiment Video
Updated: Dec 7, 2025
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
61.1K
EVI1 oncoprotein expression and CtBP1-association oscillate through the cell cycle
Roberto Paredes1,2, Marion Schneider1,2, Stella Pearson1,2
1Stem Cell and Leukaemia Proteomics Laboratory, Division of Cancer Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Molecular Biology Reports
|September 26, 2020
Summary
EVI1 protein levels and interactions change during the cell cycle in acute myeloid leukemia (AML). Understanding these dynamics is key for developing targeted therapies for EVI1-driven AML.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Aberrant EVI1 expression in acute myeloid leukemia (AML) correlates with poor patient prognosis.
- Targeted therapies for EVI1-overexpressing AML require a deeper understanding of EVI1's spatiotemporal dynamics.
- EVI1 protein interactions are crucial for its role in leukemogenesis.
Purpose of the Study:
- To investigate the cell cycle-dependent behavior of EVI1 and its shorter isoform, ΔEVI1.
- To characterize the interaction dynamics of EVI1 and ΔEVI1 with the co-repressor CtBP1.
- To determine the subcellular localization of EVI1, ΔEVI1, and CtBP1 throughout the cell cycle.
Main Methods:
- Utilized EVI1-overexpressing SB1690CB AML cells for protein quantification and interaction studies.
- Employed cell cycle synchronization using mimosine and nocodazole.
- Assessed protein expression via Western blot, immunoprecipitation, and immunofluorescence.
Main Results:
- EVI1 protein levels oscillate throughout the cell cycle and are partially degraded by the proteasome.
- Both EVI1 and ΔEVI1 interact with CtBP1, but these complexes dissociate during mitosis.
- A significant portion of EVI1, unlike ΔEVI1 or CtBP1, localizes to the nuclear matrix.
Conclusions:
- EVI1 protein levels and its interaction with CtBP1 exhibit cell cycle-dependent variations.
- Distinct differences exist in the cell cycle dynamics between EVI1 and ΔEVI1.
- These findings enhance the functional characterization of EVI1 in AML and inform targeted therapeutic strategies.
More Related Videos
12:02Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
28.1K
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
14.7K
Related Concept Videos
01:23Negative Regulator Molecules
37.7K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
37.7K
01:20The Retinoblastoma Gene
4.5K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.5K
02:34Inhibition of Cdk Activity
5.4K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.4K
02:11The Cell Cycle Control System
13.5K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
13.5K
01:28The Cell Cycle Control System
4.9K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
4.9K
02:39Positive Regulator Molecules
6.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.3K