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Pain and Activity Measurements.

David H H Molstad1, Elizabeth W Bradley2,3

  • 1Department of Orthopedics, University of Minnesota, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2020
PubMed
Summary

This study presents methods for evaluating pain and physical activity in mice for orthopedic research. These techniques aid in understanding chronic musculoskeletal pain and developing new treatments.

Keywords:
Evoked painFunctional assaysIncapacitanceMechanical allodyniaOpen-field activity assaysStatic weight bearingvon Frey

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Area of Science:

  • Orthopedics
  • Pain Research
  • Animal Models

Background:

  • Musculoskeletal pain is a leading cause of chronic pain and healthcare utilization in adults.
  • Preclinical models are essential for understanding pain mechanisms and testing interventions.
  • Standardized methods are needed for reliable assessment in orthopedic research.

Purpose of the Study:

  • To detail methods for evaluating pain and physical activity in mice.
  • To provide a framework for preclinical orthopedic studies.
  • To facilitate the study of musculoskeletal pain in animal models.

Main Methods:

  • Utilizing the von Frey filament assay to quantify mechanical allodynia (pain sensitivity).
  • Employing open-field activity assays to assess ambulation and general movement.
  • Measuring incapacitance to determine static weight-bearing distribution.

Main Results:

  • Established protocols for assessing key pain and activity parameters in mice.
  • Demonstrated the applicability of these methods in preclinical orthopedic contexts.
  • Provided quantitative data on mechanical allodynia, ambulation, and weight bearing.

Conclusions:

  • The described methods offer a robust approach for pain and activity assessment in mouse models.
  • These techniques are valuable for advancing preclinical orthopedic research.
  • This work supports the development of novel therapeutic strategies for musculoskeletal pain.