Tools and techniques for illuminating the cell biology of zinc

Zinc (Zn²⁺) is an essential micronutrient that is required for a wide variety of cellular processes. Tools and methods have been instrumental in revealing the myriad roles of Zn²⁺ in cells. This review highlights recent developments fluorescent sensors to measure the labile Zn²⁺ pool, chelators to manipulate Zn²⁺ availability, and fluorescent tools and proteomics approaches for monitoring Zn²⁺-binding proteins in cells. Finally, we close with some highlights on the role of Zn²⁺ in regulating cell function and in cell signaling.