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Nup84 persists within the nuclear envelope of the rice blast fungus, Magnaporthe oryzae, during mitosis

Mariel A Pfeifer1, Chang Hyun Khang1

  • 1Department of Plant Biology, 2502 Miller Plant Sciences, University of Georgia, Athens, GA 30602-7271, USA.

Fungal Genetics and Biology : FG & B
|September 27, 2020
PubMed

Insights

Researchers identified key nuclear envelope proteins, Nup84 and Src1, in the rice blast fungus Magnaporthe oryzae. These proteins are crucial for nuclear envelope structure and nuclear division during fungal development.

Area of Science:

  • Mycology
  • Cell Biology
  • Molecular Biology

Background:

  • The nuclear envelope's structure in the rice blast fungus, Magnaporthe oryzae, was not previously understood.
  • Understanding nuclear envelope organization is vital for fungal cell biology.

Purpose of the Study:

  • To identify and characterize components of the nuclear envelope in Magnaporthe oryzae.
  • To investigate the role of these components during nuclear division and development.

Main Methods:

  • Live-cell super-resolution structured illumination microscopy was employed.
  • Proteins Nup84 (tagged with tdTomato) and Src1 (tagged with EGFP) were visualized.

Main Results:

  • Nup84 and Src1 were found to colocalize within the nuclear envelope during interphase.
  • Nup84 remained associated with the nucleus during cell division.
  • Appressorium development included a mitotic nuclear migration event through the germ tube.

Conclusions:

  • Nup84 and Src1 are conserved components of the nuclear envelope in Magnaporthe oryzae.
  • These proteins play roles in maintaining nuclear envelope integrity and facilitating nuclear division and migration during fungal development.
Keywords:
AppressoriumIntermediate mitosisNucleoporin

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