Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Equipments Used To Measure Blood Pressure01:30

Equipments Used To Measure Blood Pressure

2.8K
Direct Method
This invasive approach involves cannulating a peripheral artery. During each cardiac contraction, pressure generates mechanical motion within the catheter, transmitted through rigid, fluid-filled tubing to a transducer. This transducer converts mechanical motion into electrical signals displayed as waveforms on a monitor. An automatic flushing system prevents blood backflow. Due to the potential risk of unexpected arterial blood loss, this method is primarily used in intensive...
2.8K
Measurement of Fluid Pressure01:16

Measurement of Fluid Pressure

466
Fluid pressure is commonly measured using devices called manometers, which rely on liquid columns to indicate pressure differences. The height of a liquid column in a manometer reflects the pressure exerted by the fluid, providing a simple yet effective means of measurement. Different types of manometers serve specific purposes based on their configurations and the type of fluids involved.
A basic form of manometer is the piezometer, a vertical tube open at the top and filled with the same...
466

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.

bioRxiv : the preprint server for biology·2025
Same author

Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells.

European cells & materials·2016
Same author

Form and function of the intervertebral disc in health and disease: a morphological and stain comparison study.

Journal of anatomy·2014
Same author

Fibrin-genipin adhesive hydrogel for annulus fibrosus repair: performance evaluation with large animal organ culture, in situ biomechanics, and in vivo degradation tests.

European cells & materials·2014
Same author

Development and validation of a bioreactor system for dynamic loading and mechanical characterization of whole human intervertebral discs in organ culture.

Journal of biomechanics·2014
Same author

Time-resolved measurements of individual ion cloud signals to investigate space-charge effects in plasma mass spectrometry.

Journal of the American Society for Mass Spectrometry·2013

Related Experiment Video

Updated: Dec 7, 2025

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.0K

A method for measuring intra-tissue swelling pressure using a needle micro-osmometer.

C M Krull, A D Lutton, J W Olesik

  • 1Department of Biomedical Engineering, The Ohio State University, Mars G. Fontana Laboratories, 140 W. 19th Ave, Room 3155, Columbus, OH 43210, USA.walter.367@osu.edu.

European Cells & Materials
|September 27, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel micro-osmometer technique to measure intervertebral disc swelling pressure and osmolality. The method accurately quanties osmotic pressures, revealing limitations in current models for disc health and disease.

More Related Videos

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
09:06

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Published on: July 14, 2022

1.9K
Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

3.0K

Related Experiment Videos

Last Updated: Dec 7, 2025

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.0K
Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
09:06

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Published on: July 14, 2022

1.9K
Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

3.0K

Area of Science:

  • Biomedical Engineering
  • Tissue Mechanics
  • Physiology

Background:

  • Intervertebral disc function relies on osmotic swelling pressures.
  • Diurnal and disease-related osmotic changes influence cellular activity.
  • In vivo osmotic environments within the disc remain poorly understood.

Purpose of the Study:

  • To demonstrate a micro-osmometer technique for measuring intra-tissue swelling pressure and osmolality.
  • To compare flux-based swelling pressure measurements with ionic swelling pressures predicted by Gibbs-Donnan theory in nucleus pulposus (NP) tissue.

Main Methods:

  • Developed a micro-osmometer fluid flux model based on Darcy's law.
  • Applied varying pressures (0.03-0.57 MPa) to NP tissue using equilibrium dialysis.
  • Measured intra-tissue swelling pressures via flux and ionic swelling pressures using ICP-OES and Gibbs-Donnan calculations.

Main Results:

  • Achieved high concordance (0.93) between applied pressures and flux-based swelling pressure measurements.
  • Determined effective tissue osmolality bounds of 376-522 mOsm/kg H2O under simulated diurnal loading.
  • Identified significant differences between flux and Gibbs-Donnan measures, highlighting non-ionic contributions and the need for tissue water normalization.

Conclusions:

  • The micro-osmometer technique provides a viable method for measuring intra-tissue swelling pressure and osmolality.
  • Standard constitutive models may underestimate intra-tissue swelling pressure due to unaddressed factors.
  • This technique can advance understanding of diurnal osmotic variations and mechanotransduction in disc health and disease.