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Near-Infrared Fluorescence MOF Nanoprobe for Adenosine Triphosphate-Guided Imaging in Colitis
Yi-Ting Zhao1, Xi-Xi Chen1, Wen-Li Jiang1
1Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, P. R. China.
Abstract:
Adenosine triphosphate (ATP) is mainly produced in mitochondria and plays an important role in lots of pathological processes such as colitis. Unfortunately, to date, few suitable fluorescence probes have been developed for monitoring the ATP level in colitis. Herein, a fluorescence nanoprobe named NIR@ZIF-90 is proposed and prepared by encapsulating a rhodamine-based near-infrared (NIR) dye into zeolitic imidazolate frameworks (ZIF-90). The nanoprobe is nonfluorescent because the emission of NIR is suppressed by the encapsulation, while in the presence of ATP, the framework of ZIF-90 is dissembled to release NIR and thus NIR fluorescence at 750 nm is observed. The nanoprobe shows high sensitivity to ATP with a 72-fold increase and excellent selectivity to ATP over other nucleotides. Moreover, with low cytotoxicity and good mitochondria-targeted ability, NIR@ZIF-90 is used to image ATP in colorectal cancer cells (HCT116). In addition, due to the NIR emission, the nanoprobe is further employed to successfully monitor the ATP level in a colitis mouse model. To the best of our knowledge, the nanoprobe is the first example to study colitis in vivo with the guidance of ATP, which will provide an efficient tool for understanding colitis.
Insights
Researchers developed a novel fluorescence nanoprobe, NIR@ZIF-90, to detect adenosine triphosphate (ATP) in colitis. This tool enables in vivo monitoring of ATP levels in a colitis mouse model, advancing colitis research.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Imaging
Background:
- Adenosine triphosphate (ATP) is crucial in mitochondrial function and pathological processes like colitis.
- Current methods for monitoring ATP in colitis are limited, hindering research and understanding.
Purpose of the Study:
- To develop and validate a novel fluorescence nanoprobe for sensitive and selective detection of ATP.
- To investigate the potential of this nanoprobe for imaging ATP in colorectal cancer cells and a colitis mouse model.
Main Methods:
- Synthesis of NIR@ZIF-90 nanoprobe by encapsulating a near-infrared (NIR) dye within zeolitic imidazolate frameworks (ZIF-90).
- Characterization of the nanoprobe's fluorescence response to ATP, including sensitivity, selectivity, and cytotoxicity.
- In vitro imaging of ATP in HCT116 colorectal cancer cells and in vivo monitoring in a colitis mouse model.
Main Results:
- The NIR@ZIF-90 nanoprobe exhibited a 72-fold fluorescence increase in response to ATP, demonstrating high sensitivity and selectivity.
- The nanoprobe showed low cytotoxicity and effective mitochondria-targeting capabilities.
- Successful in vivo monitoring of ATP levels in a colitis mouse model was achieved using the NIR@ZIF-90 nanoprobe.
Conclusions:
- NIR@ZIF-90 is a sensitive and selective fluorescence nanoprobe for ATP detection.
- This nanoprobe provides a novel tool for in vivo ATP imaging in colitis, offering new insights into the disease.
- This represents the first use of an ATP-guided nanoprobe for in vivo colitis studies.
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