Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

4.5K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Abnormal Proliferation02:23

Abnormal Proliferation

5.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Tumor Progression02:07

Tumor Progression

7.0K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.0K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

4.5K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.5K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

10.7K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

4.9K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Information quality, readability, and empathy of AI-generated public mental health information: a comparative evaluation of eight large language models.

Frontiers in public health·2026
Same author

2025 Update of the Taiwan Expert Consensus on Hormone Receptor-Positive Metastatic Breast Cancer Management.

Journal of breast cancer·2026
Same author

Modular Dearomative 1,4-Addition to Simple Arenes with Dual Nucleophiles: Overriding the Inherent 1,2-Selectivity via η<sup>6</sup>-Coordination.

Journal of the American Chemical Society·2026
Same author

Integration of multi-omics for precision therapy in breast cancer.

Drug discovery today·2026
Same author

ZBTB18-mediated STAT1 transcriptional repression contributes to bovine myogenesis, implying an association with oxidative myofiber formation and beef eating quality.

Food chemistry. Molecular sciences·2026
Same author

A Comparative Study of Rapid Fresh Pathology Imaging and Standard FFPE H&E Histopathology: A High Concordance in the Evaluation of Lung and Breast Cancer.

Diagnostics (Basel, Switzerland)·2026

Related Experiment Video

Updated: Dec 7, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K

circKRT7-miR-29a-3p-COL1A1 Axis Promotes Ovarian Cancer Cell Progression.

Qiang An1, Ting Liu1, Ming-Yang Wang1

  • 1Department of Gynecology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People's Republic of China.

Oncotargets and Therapy
|September 28, 2020
PubMed
Summary

Circular RNA KRT7 (circKRT7) promotes ovarian cancer progression by sponging miR-29a-3p, leading to COL1A1 upregulation. Inhibiting circKRT7 suppressed tumor growth and metastasis in ovarian cancer models.

Keywords:
EMTKRT7circular RNAovarian cancer

More Related Videos

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

15.5K
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

14.1K

Related Experiment Videos

Last Updated: Dec 7, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

15.5K
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

14.1K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are key regulators in human disease progression.
  • The specific roles of circRNAs in ovarian cancer are not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of circKRT7 in ovarian cancer.
  • To explore the regulatory network involving circKRT7, miR-29a-3p, and COL1A1 in ovarian cancer.

Main Methods:

  • Identified circKRT7 expression using DNA sequencing and PCR.
  • Verified targeting relationships via fluorescence reporter assays.
  • Assessed proliferation, migration, and invasion in vitro and tumor growth in vivo.

Main Results:

  • circKRT7 promotes ovarian cancer cell proliferation and metastasis by sponging miR-29a-3p, upregulating COL1A1.
  • Knockdown of circKRT7 inhibited ovarian cancer cell migration and invasion in vitro.
  • Reduced circKRT7 expression slowed tumor growth in vivo, with partial rescue by miR-29a-3p inhibition.

Conclusions:

  • circKRT7 promotes epithelial-mesenchymal transition (EMT)-related progression in ovarian cancer by sponging miR-29a-3p.
  • circRNAs play a critical role in the malignant evolution of ovarian cancer.