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Theabrownin Induces Apoptosis and Tumor Inhibition of Hepatocellular Carcinoma Huh7 Cells Through ASK1-JNK-c-Jun

Jiaan Xu1,2, Bo Yan1,2, Lei Zhang3

  • 1College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.

Oncotargets and Therapy
|September 28, 2020
PubMed
Abstract

Insights

Theabrownin (TB) effectively inhibits hepatocellular carcinoma (HCC) cell growth and promotes apoptosis by activating the JNK signaling pathway. This study reveals TB

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Theabrownin (TB), a key tea pigment, exhibits anti-tumor properties.
  • The effects of TB on hepatocellular carcinoma (HCC) are not well understood.

Purpose of the Study:

  • To investigate the anti-cancer effects of TB on HCC cells.
  • To elucidate the molecular mechanisms underlying TB's action in HCC.

Main Methods:

  • In vitro studies using Huh7 HCC cells assessed TB's effects on cell viability and apoptosis.
  • Molecular analyses included real-time PCR and Western blotting to examine gene and protein expression.
  • In vivo efficacy was evaluated using a zebrafish xenograft model, with JNK pathway inhibition used to confirm mechanism.

Main Results:

  • TB demonstrated significant dose-dependent anti-proliferative and pro-apoptotic effects on Huh7 cells.
  • TB modulated key apoptosis-related genes (NOXA, PUMA, P21, Bax, Bim) and proteins (ASK-1, Bax, p-JNK, p-c-Jun, Bcl-2).
  • In vivo, TB exhibited potent tumor inhibition, surpassing cis-platinum, via JNK pathway activation.

Conclusions:

  • Theabrownin (TB) effectively inhibits hepatocellular carcinoma (HCC) cell proliferation and induces apoptosis.
  • TB's anti-cancer activity is mediated through the activation of the JNK signaling pathway.
  • This study provides novel evidence for TB's therapeutic potential against HCC.
Keywords:
JNKapoptosishepatocellular carcinomatheabrowninzebrafish

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