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Caspase Recruitment Domain-Containing Protein 9 Expression is a Novel Prognostic Factor for Lung Adenocarcinoma
Nanako Miwa1, Tatsuya Nagano1, Naoe Jimbo2
1Division of Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
Insights
High expression of Caspase recruitment domain-containing protein 9 (CARD9) in lung adenocarcinoma indicates a poor prognosis. CARD9 may be a therapeutic target for lung cancer treatment.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Caspase recruitment domain-containing protein 9 (CARD9) is vital for innate immunity and implicated in tumor progression.
- Limited research exists on CARD9's specific role in lung cancer development and outcomes.
Purpose of the Study:
- To investigate the role and prognostic significance of CARD9 in lung adenocarcinoma.
- To explore CARD9's functional impact on lung adenocarcinoma cell behavior.
Main Methods:
- Immunohistochemical analysis of CARD9 expression in 74 lung adenocarcinoma tumor samples.
- Functional assays using CARD9 siRNA in lung adenocarcinoma cell lines to assess proliferation, apoptosis, and pathway activation.
Main Results:
- High CARD9 expression was detected in 32.4% of tumors and correlated with significantly poorer overall survival (P=0.0365).
- High CARD9 expression was identified as an independent prognostic factor for adverse outcomes in lung adenocarcinoma.
- CARD9 knockdown inhibited lung adenocarcinoma cell proliferation without affecting apoptosis and activated the NF-κB pathway.
Conclusions:
- CARD9 serves as an independent prognostic marker for poor outcomes in lung adenocarcinoma.
- CARD9's involvement in proliferation and NF-κB activation suggests its potential as a therapeutic target for lung cancer.
Purpose:
Caspase recruitment domain-containing protein 9 (CARD9) is expressed at high levels in bone marrow cells and has a crucial role in innate immunity. Current studies indicate that CARD9 also plays a key role in tumor progression, but there are few reports on the role of CARD9 in lung cancer. The aim of this study was to clarify the role of CARD9 in lung adenocarcinoma.
Patients And Methods:
Lung adenocarcinoma tumor samples from 74 patients who underwent complete resection at Kobe University Hospital from January 2014 to December 2014 were analyzed by immunohistochemistry. The role of CARD9 in cancer cells was analyzed using lung cancer cell lines treated with CARD9 siRNA.
Results:
High expression of CARD9 was observed in 32.4% of tumors, and compared to low expression of CARD9, high expression was associated with poorer overall survival (P = 0.0365). Univariate and multivariate analyses showed that high expression of CARD9 was an independent prognostic factor. Knockdown of CARD9 in lung adenocarcinoma cells inhibited proliferation but did not increase apoptosis. In addition, CARD9 activated the NF-κB pathway in a lung adenocarcinoma cell line.
Conclusion:
CARD9 was shown to be an independent prognostic factor of poor outcome for lung cancer and may represent a molecular target for treatment.
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