Related Experiment Video

Updated: Dec 7, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

756

MicroRNA-182 Promotes Cell Migration by Targeting Programmed Cell Death 4 in Hepatocellular Carcinoma Cells

Junwei Hu1,2, Zeyu Wang1, Jinjun Wang3

  • 1Department of Gastroenterology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, People's Republic of China.

Oncotargets and Therapy
|September 28, 2020
PubMed
Abstract

Insights

MicroRNA-182 (miR-182) promotes liver cancer cell migration by downregulating the tumor suppressor programmed cell death 4 (PDCD4). This study confirms miR-182 targets PDCD4 in hepatocellular carcinoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Hepatology

Background:

  • Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality globally.
  • Programmed cell death 4 (PDCD4) is a known tumor suppressor in liver cancer.
  • Mechanisms regulating PDCD4 expression in HCC remain largely unelucidated.

Purpose of the Study:

  • Investigate PDCD4 and miR-182 expression in HCC.
  • Determine if PDCD4 is a direct target of miR-182 in HCC cell lines.
  • Elucidate the role of miR-182 in hepatocarcinogenesis.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blotting for expression analysis.
  • Transwell and wound healing assays to assess cell migration.
  • Luciferase reporter assay to confirm direct target interaction.

Main Results:

  • PDCD4 expression was downregulated, while miR-182 was upregulated in HCC cell lines and tissues.
  • miR-182 significantly promoted migration of liver cancer cells.
  • PDCD4 was confirmed as a direct target of miR-182, with inverse expression correlation.

Conclusions:

  • miR-182 functions as an oncogene in HCC.
  • miR-182 promotes liver cancer progression by directly downregulating PDCD4.
  • Targeting the miR-182/PDCD4 axis may offer therapeutic strategies for HCC.
Keywords:
PDCD4hepatocellular carcinomamiR-182migration

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.5K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K