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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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CircTTBK2 Contributes to the Progression of Glioma Through Regulating miR-145-5p/CPEB4 Axis

Yan Liu1, Ruilong Li1, Xuan Wang2

  • 1Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin 300052, People's Republic of China.

Cancer Management and Research
|September 28, 2020
PubMed
Abstract

Insights

Circular RNA TTBK2 (circTTBK2) promotes glioma progression by sponging miR-145-5p and upregulating CPEB4. Inhibiting circTTBK2 may offer a novel therapeutic strategy for glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various cancers, including glioma.
  • The specific role of circTTBK2 in glioma pathogenesis remains largely unknown.

Purpose of the Study:

  • To investigate the function and mechanism of circTTBK2 in glioma.
  • To explore circTTBK2 as a potential therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot for gene and protein expression.
  • Cellular assays (MTT, flow cytometry, Transwell) for proliferation, apoptosis, and metastasis.
  • In vivo murine xenograft models to assess tumorigenesis.
  • Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.

Main Results:

  • CircTTBK2 expression is elevated in glioma tissues and associated with poor patient survival.
  • CircTTBK2 knockdown inhibits glioma cell proliferation, migration, invasion, and glycolysis, while promoting apoptosis.
  • CircTTBK2 acts as a molecular sponge for miR-145-5p, and miR-145-5p targets CPEB4.
  • CircTTBK2 promotes glioma progression via the miR-145-5p/CPEB4 axis.

Conclusions:

  • CircTTBK2 functions as an oncogenic circRNA in glioma by regulating the miR-145-5p/CPEB4 pathway.
  • CircTTBK2 represents a promising therapeutic target for glioma treatment.
Keywords:
CPEB4circTTBK2gliomamiR-145-5p

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