Growth inhibition of mastitis pathogens by long-chain fatty acids

Staphylococcus aureus, Staphylococcus hyicus, Streptococcus agalactiae, and Corynebacterium bovis were tested for sensitivity to long-chain fatty acids predominant in teat canal keratin. Antibacterial activity of free fatty acids on each bacterial species was measured after 12 and 24 h in chemically defined media. Polyene C18:2 and C18:3 acids were bactericidal to each species at less than or equal to 10(5) ng/ml-1. The most bacteriostatic saturated fatty acids were C12 and C14. Streptococcus agalactiae growth was inhibited more by fatty acids after 24 h than after 12 h. No incubation time effect on growth responses of other species was determined. Polyoxyethylenesorbitan monooleate had a neutralizing effect on the bactericidal activity of polyene acids on C. bovis. Corynebacterium bovis were unable to grow in synthetic media containing individual free fatty acids as the sole source of preformed fatty acids. A relationship between bacterial species commonly isolated from bovine teat canals with resistance to fatty acids predominant in keratin was not evident.