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Related Experiment Videos

Interactions between fibroblasts and a reconstituted basement membrane matrix.

H Emonard, A Calle, J A Grimaud

    The Journal of Investigative Dermatology
    |August 1, 1987
    PubMed
    Summary
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    Normal calf skin fibroblasts remodel basement membrane matrix, altering their own cell structure. This interaction reveals how cells and their extracellular matrix dynamically influence each other in tissue environments.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Tissue Engineering

    Background:

    • Basement membranes are crucial extracellular matrices providing structural support and signaling cues.
    • Understanding fibroblast interactions with basement membranes is key to tissue repair and regeneration.

    Purpose of the Study:

    • To investigate the dynamic interactions between normal calf skin fibroblasts and a reconstituted basement membrane matrix.
    • To elucidate how fibroblasts remodel the basement membrane and how this affects their ultrastructure.

    Main Methods:

    • Utilized a gel-like reconstituted basement membrane matrix composed of type IV collagen, laminin, entactin, nidogen, and heparan sulfate proteoglycan.
    • Employed cell culture techniques and electron microscopy to observe fibroblast behavior and ultrastructural changes over time.

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    Main Results:

    • Fibroblasts migrated and formed a cellular network on the matrix within 24 hours.
    • Fibroblasts remodeled the matrix by day 3, penetrating the gel, depositing fibronectin and collagen, and retracting extracellular matrix.
    • Cultured fibroblasts exhibited altered ultrastructure, including a reduced synthetic apparatus, enlarged cytoskeleton, and intracytoplasmic laminin vesicles.

    Conclusions:

    • Reconstituted basement membrane matrix is actively remodeled by skin fibroblasts.
    • Fibroblast ultrastructure is reciprocally influenced by their interaction with the basement membrane matrix.