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Updated: Dec 7, 2025

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Published on: July 6, 2022
Chlorophyll a fluorescence kinetics of mung bean (Vigna radiata L.) grown under artificial continuous light
Deepak Kumar1, Hanwant Singh1, Shani Raj1
1Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, 313001, Rajasthan, India.
Abstract:
Continuous light can be used as a tool to understand the diurnal rhythm of plants and it can also be used to increase the plant production. In the present research, we aimed to investigate the photosynthetic performance of V. radiata under continuous light as compared with the plants grown under normal light duration. Chlorophyll a fluorescence transient (OJIP test) technique was used to understand the effect on various stages of photosynthesis and their consequences under continuous light condition. Various Chl a Fluorescence kinetic parameters such as Specific energy fluxes (per QA-reducing PSII reaction center (RC)) (ABS /RC; TR0/RC; ET0/RC; DI0/RC), phenomenological fluxes, leaf model, (ABS/CSm; TR/CSm; ETo/CSm), Quantum yields and efficiencies (φPo; φEo; Ψo) and Performance index (PIabs) was extracted and analysed in our investigation. Conclusively, our study has revealed that continuous light alters the photosynthetic performance of V. radiata at a different point but also improve plant productivity.
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