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Endocytosis in the embryonic chick chorionic epithelium
The Journal of Experimental Zoology. Supplement : Published Under Auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry
|January 1, 1987
Summary
Chick embryo chorionic epithelium cells, villus cavity (VC) and sinus-covering (SC) cells, exhibit endocytic activity. This process may aid in recovering water and ions from the eggshell during development.
Area of Science:
- Developmental Biology
- Cell Biology
- Embryology
Background:
- The developing chick embryo's chorionic epithelium comprises villus cavity (VC) and sinus-covering (SC) cells.
- VC cells feature apical microvilli with vesicles, while SC cells have thin cytoplasmic processes overlying blood sinuses.
Purpose of the Study:
- To investigate if vesicles in VC and SC cells are endocytic.
- To determine if endocytosis occurs in both VC and SC cell types.
Main Methods:
- Exposure of in ovo chorionic epithelium to microperoxidase (MRP-11).
- Exposure of shell-less cultured embryos' chorionic epithelium to horseradish peroxidase (HRP).
- Ultrastructural analysis to detect endocytic uptake of markers.
Main Results:
- MRP-11 uptake observed in 70% of SC cells and 20% of VC cells in ovo.
- HRP uptake observed in 80% of SC cells and 33% of VC cells in shell-less culture.
- Both VC and SC cells demonstrate endocytic activity under both experimental conditions.
Conclusions:
- VC and SC cells actively engage in endocytosis.
- In ovo endocytosis by these cells may facilitate water and ion recovery from the eggshell and membranes.