Related Experiment Video
Updated: Dec 7, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Over-expression of rice R1-type MYB transcription factor confers different abiotic stress tolerance in transgenic
Poonam Tiwari1, Yuvraj Indoliya2, Abhishek Singh Chauhan2
1Molecular Biology and Biotechnology Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India; Department of Biotechnology, Kumaun University, Nainital, 26300, India.
Abstract:
Among various abiotic stresses, water deficit hit the first in the list followed by heavy metal stresses as a serious environmental growth-limiting factor that restricts the global crop yield. Molecular approaches will help us to trace key regulators which are involved in stress-related phenomenon to enhance crop productivity. The present study functionally characterized one of the key regulators, OsMYB-R1 in Arabidopsis. Phylogenetic analyses indicated that OsMYB-R1 had a close relationship with Sorghum bicolour and Zea mays. Ectopic expression of OsMYB-R1 in Arabidopsis resulted in improved tolerance to PEG/drought and chromium stress in addition to conferring no tolerance to salinity stress. Further RNA seq. data revealed that OsMYB-R1 regulates the expression of key genes that improve the root architecture and maintain the cellular homeostasis of transgenic lines through an efficient anti-oxidant system. It also reveals the differential gene expression of stress-responsive and hormone-responsive genes, which indicate the intricate network of defense regulatory machinery activated in transgenic lines. Additionally, salicylic acid (SA) plays a significant role in promoting the growth of the OsMYB-R1 over-expressing plants and increased GUS intensity in SA treated OsMYB-R1 promoter plants demonstrate the explicit role of SA signaling in overcoming stress tolerance. Whereas no significant change was observed in OsMYB-R1 over-expressing plants after ABA and MeJA treatment. Overall, OsMYB-R1 is a promising gene resource for improving abiotic stress tolerance in other crops, especially in dicotyledon plants.
More Related Videos
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
11:27A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
Published on: August 25, 2018
Related Concept Videos
Responses to Drought and Flooding
Responses to Salt Stress
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Cell Signaling in Plants
Plant Breeding and Biotechnology