Related Experiment Video
Updated: Dec 7, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A carbon dot-based fluorometric probe for oxytetracycline detection utilizing a Förster resonance energy transfer
Yanzhao Fu1, Li Huang1, Shaojing Zhao2
1Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Shenzhen Research Institute of Central South University, Shenzhen 518057, PR China.
Abstract:
A carbon dot (CD)-based fluorometric probe for oxytetracycline (OTC) detection utilizing a Förster resonance energy transfer (FRET) mechanism was firstly developed. Upon addition of OTC, the blue fluorescence of the CDs peaked at 405 nm was quickly quenched, with a new fluorescence peaked at 505 nm attributed to OTC was observed within 30 s. A visual fluorescent color change of the CDs solution from blue to green was discerned under a 365 nm UV light irradiation. The CDs displayed a high sensitivity and selectivity toward OTC with a low detection limit of 0.41 μM. Furthermore, the probe was applied to detect OTC in water, milk, and pork samples with a satisfied recovery.
More Related Videos
Related Concept Videos
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Fluorescence and Phosphorescence: Instrumentation

