A novel ratiometric fluorescent probe for selective detection and imaging of H₂S

In this work, a novel phenoxazine-based fluorescent probe BPO-N₃ was developed to detect H₂S. The results showed that the probe had high selectivity and sensitivity toward H₂S, and its detection mechanism was based the ratio between green and red fluorescence signals; its detection limit was as low as 30 nM. The fluorescent imaging experiments further showed that the probe BPO-N₃ could successfully detect endogenous and exogenous H₂S in living cells. This probe can be used as a powerful tool for in-depth study of H₂S function in various physiological processes.