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Decellularization and Recellularization of Whole Livers
Published on: February 4, 2011
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Development of highly functional bioengineered human liver with perfusable vasculature
Da-Hyun Kim1, Jungho Ahn2, Hyun Kyoung Kang1
1Adult Stem Cell Research Center and Research Institute for Veterinary Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Biomaterials
|September 28, 2020
Summary
This study presents a new method for creating vascularized bioengineered human livers (VBHL) using aptamer-coated scaffolds. This approach significantly reduces blood clotting and improves liver function after transplantation in a rat model.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Vascular Biology
Background:
- Liver failure necessitates innovative treatments beyond transplantation.
- Vascularization is critical for engineered organs to prevent rejection and thrombosis.
- Current bioengineering strategies face challenges in achieving functional vascular networks.
Purpose of the Study:
- To develop an efficient strategy for reconstructing a vascularized bioengineered human liver (VBHL).
- To enhance the integration and function of engineered liver constructs through improved vascularization.
- To reduce the risk of post-transplantation complications like thrombosis.
Main Methods:
- Decellularized liver scaffolds were coated with anti-CD31 aptamers for enhanced re-endothelialization.
- Ex vivo human blood perfusion was used to assess anti-thrombotic properties.
- Liver parenchymal and nonparenchymal cells were seeded to form VBHL constructs.
- In vivo studies evaluated vascular network formation and liver function after heterotopic transplantation.
Main Results:
- Anti-CD31 aptamer coating significantly reduced platelet aggregation in scaffolds.
- VBHL constructs exhibited organized liver-like structures with aligned vasculature.
- Constructs demonstrated enhanced long-term liver-specific functions.
- In vivo studies confirmed perfusable vessel networks and direct vascular connections.
- Transplanted VBHL constructs supported liver function in a rat model of liver fibrosis.
Conclusions:
- A novel and efficient strategy for creating VBHL with functional vascular reconstruction was established.
- Aptamer-mediated vascularization is a promising approach to improve the viability and function of engineered organs.
- This method offers a potential solution for generating transplantable bioengineered livers to treat liver failure.
