First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D₁ (former D_1A), D₅ (former D_1B), D₆ (former D_1C and D_1D) and D₇ (former D_1E), while the D2 family encloses five subtypes, D₂, D₃, D₄, D₈ (former D_2like or D_2l) and D₉ (former D_{4-related sequence} or D_4-rs). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D1 family: two D₁, one D_5a, one D_6a and one D_6b and to the D2 family: two spliced variants of D₂, one D₃, one D₈ and one D_9. Unlike zebrafish, the subtypes D₄ and D₇ have not yet been isolated in pikeperch. As expected D₁, D₃, D₈ and D₉ are mostly expressed in brain parts except for the cerebellum (D₁ and D₃). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.