Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.2K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.2K
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

3.1K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
3.1K
Photoluminescence: Applications01:14

Photoluminescence: Applications

884
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
884
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Valorization of Vine Shoot Waste into Phenolic-Rich Liquors for Laccase-Mediated Functionalization of Starch.

Foods (Basel, Switzerland)·2026
Same author

Cultivar-Specific Differences in C6 and C7 Sugar Metabolism During Avocado Ripening: Comparative Insights from <i>Bacon</i>, <i>Fuerte</i>, and <i>Hass</i>.

Plants (Basel, Switzerland)·2025
Same author

Multi-response deconvolution of auditory evoked potentials in a reduced representation space.

The Journal of the Acoustical Society of America·2024
Same author

Subspace-constrained deconvolution of auditory evoked potentials.

The Journal of the Acoustical Society of America·2022
Same author

Luminescent biomimetic citrate-coated europium-doped carbonated apatite nanoparticles for use in bioimaging: physico-chemistry and cytocompatibility.

RSC advances·2022
Same author

Luminescent Citrate-Functionalized Terbium-Substituted Carbonated Apatite Nanomaterials: Structural Aspects, Sensitized Luminescence, Cytocompatibility, and Cell Uptake Imaging.

Nanomaterials (Basel, Switzerland)·2022
Same journal

RETRACTED: Zhang et al. A Novel Framework for Reconstruction and Imaging of Target Scattering Centers via Wide-Angle Incidence in Radar Networks. <i>Sensors</i> 2025, <i>25</i>, 6802.

Sensors (Basel, Switzerland)·2026
Same journal

Enhancing Unsupervised Multi-Source Domain Adaptation for Person Re-Identification via Mixture of Experts and Graph-Based Relation.

Sensors (Basel, Switzerland)·2026
Same journal

Development of an Instrumented Glove for Palmar Pressure Assessment in Kayakers.

Sensors (Basel, Switzerland)·2026
Same journal

Development and Experimental Validation of an Autonomous IoT-Based Monitoring System for Real-Time Water Quality Assessment in the Amazon River.

Sensors (Basel, Switzerland)·2026
Same journal

Semi-Supervised Adversarial Learning Framework for Controller Area Network Bus Intrusion Detection.

Sensors (Basel, Switzerland)·2026
Same journal

Smart Optimization Method for Safety Signs in Innovative Manufacturing Environments Integrating Industrial Field IoT Sensors and Knowledge Graphs.

Sensors (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Dec 7, 2025

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.8K

Self-Referenced Multifrequency Phase-Resolved Luminescence Spectroscopy.

Angel de la Torre1, Santiago Medina-Rodríguez2, Jose C Segura1

  • 1Department of Signal Theory, Networking and Communications, University of Granada, 18071 Granada, Spain.

Sensors (Basel, Switzerland)
|September 29, 2020
PubMed
Summary
This summary is machine-generated.

A new self-referenced method estimates luminescence lifetimes using only the emission signal, simplifying sensor design and improving robustness. This technique is applicable to various luminescence chemical sensors, including oxygen measurement systems.

Keywords:
chemical sensorfrequency responseluminescence spectroscopymultifrequencyoxygen sensingquadrature detectionself-referenced analysis

More Related Videos

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
08:35

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

9.6K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.2K

Related Experiment Videos

Last Updated: Dec 7, 2025

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.8K
Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
08:35

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

9.6K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.2K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemical Sensing

Background:

  • Phase-resolved luminescence sensors determine analytes by measuring luminescence lifetime.
  • Luminescence lifetime is typically calculated using both excitation and emission signals.
  • Conventional methods require complex instrumentation to record both signals.

Purpose of the Study:

  • To introduce a novel self-referenced method for estimating luminescence lifetimes.
  • To simplify the instrumentation architecture for luminescence chemical sensors.
  • To enhance the robustness of luminescence lifetime measurements.

Main Methods:

  • Developed a theoretical framework for deriving luminescence lifetimes solely from the emission signal.
  • Utilized the harmonics present in the emission signal as an internal reference.
  • Applied the method to an oxygen-sensing system for validation.

Main Results:

  • Demonstrated that luminescence lifetime can be accurately determined from the emission signal alone when it contains at least two harmonics.
  • The proposed method simplifies instrument design by eliminating the need for a separate excitation signal reference.
  • Self-referenced lifetime estimation enhances robustness against sensor degradation and optical coupling variations.

Conclusions:

  • The novel self-referenced method offers practical advantages for luminescence chemical sensors, including simplified hardware and improved reliability.
  • This approach reduces the need for frequent recalibration, making sensors more user-friendly.
  • The technique holds promise for advancing the field of optical chemical sensing.