Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serum bactericidal testing with quality control organisms.

L N Slater, D J Flournoy

    Methods and Findings in Experimental and Clinical Pharmacology
    |April 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Prevalence, risk factors, and genetic diversity of Bartonella henselae infections in pet cats in four regions of the United States.

    Journal of clinical microbiology·2004
    Same author

    Does rinsing the mouth before expectoration improve sputum specimen quality?

    Chest·2002
    Same author

    Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a species-specific PCR diagnostic assay.

    Journal of clinical microbiology·2001
    Same author

    Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in persons coinfected with human immunodeficiency virus.

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2001
    Same author

    Isolation of fungi from rose bush thorns.

    The Journal of the Oklahoma State Medical Association·2000
    Same author

    Increasing antimicrobial resistance in gram-negative bacilli isolated from patients in intensive care units.

    American journal of infection control·2000
    Same journal

    [Proceedings of the XVIII TASPE Seminar, Granada, Spain, 4-5 February 2010].

    Methods and findings in experimental and clinical pharmacology·2011
    Same journal

    [Treatment algorithm for gastrointestinal graft-versus-host disease].

    Methods and findings in experimental and clinical pharmacology·2011
    Same journal

    [Treatment of chronic graft-versus-host disease with protein tyrosine kinase inhibitors].

    Methods and findings in experimental and clinical pharmacology·2011
    Same journal

    [Classification of chronic graft-versus-host disease].

    Methods and findings in experimental and clinical pharmacology·2011
    Same journal

    [Pegfilgrastim in hematopoietic stem cell transplantation].

    Methods and findings in experimental and clinical pharmacology·2011
    Same journal

    [Allogeneic transplantation in multiple myeloma patients: results and recommendations in February 2010].

    Methods and findings in experimental and clinical pharmacology·2011
    See all related articles

    This study compared serum bacteriostatic and bactericidal titers using quality control organisms. Establishing acceptable ranges for these assays can improve accuracy and reduce variations in results.

    Area of Science:

    • Microbiology
    • Clinical Chemistry

    Background:

    • Accurate measurement of serum antimicrobial activity is crucial for effective patient treatment.
    • Variability in serum bactericidal assay (SBA) results can complicate clinical decision-making and inter-laboratory comparisons.

    Purpose of the Study:

    • To compare measurements of serum bacteriostatic and bactericidal titers.
    • To establish acceptable ranges for serum bactericidal assay results.
    • To improve the accuracy and reduce variability of SBA.

    Main Methods:

    • Serum samples were tested against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922.
    • Antimicrobial agents (oxacillin, vancomycin, gentamicin, ampicillin) were added to sera for testing.
    • Duplicate testing was performed to assess reproducibility.

    Related Experiment Videos

    Main Results:

    • Specific acceptable ranges for serum bacteriostatic and bactericidal titers were determined for the tested organisms and antibiotics.
    • The study provides data for quality control in antimicrobial susceptibility testing.

    Conclusions:

    • Establishing acceptable ranges for SBA results is recommended.
    • Standardized ranges can enhance data comparability across studies and reduce intralaboratory variations.
    • Improved accuracy and consistency in SBA are vital for reliable antimicrobial susceptibility testing.