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A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis
Bénédicte Desvoyes1, Ainhoa Arana-Echarri2, María D Barea2
1Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, Madrid, Spain. bdesvoyes@cbm.csic.es.
Nature Plants
|September 29, 2020
Summary
Researchers developed PlaCCI, a plant cell cycle indicator, to track cell division during organ development. This fluorescent sensor enables robust live imaging and quantification of cell cycle phases in Arabidopsis thaliana.
Area of Science:
- Plant biology
- Developmental biology
- Cell biology
Background:
- Understanding organogenesis requires assessing cell proliferation dynamics.
- Spatiotemporal control of cell division is key to plant development.
Purpose of the Study:
- To develop a versatile fluorescent sensor for identifying plant cell cycle phases.
- To enable robust live imaging and quantification of cell cycle progression in Arabidopsis thaliana.
Main Methods:
- Generated a fluorescent sensor, PlaCCI (plant cell cycle indicator), using CDT1a-CFP, H3.1-mCherry, and CYCB1;1-YFP markers.
- Integrated all markers and antibiotic resistance into a single expression cassette for easier use in mutant backgrounds.
- Utilized live-imaging experiments to validate the sensor's performance.
Main Results:
- PlaCCI successfully identifies distinct cell cycle phases in Arabidopsis thaliana.
- The sensor functions effectively across various plant organs.
- The single-cassette system facilitates selection in different genetic backgrounds.
Conclusions:
- PlaCCI is a robust tool for studying plant cell proliferation dynamics.
- This indicator line is valuable for live imaging and quantifying cell cycle progression during organogenesis.
