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Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites.

Mariana B Méndez1,2, Jorge A Trelles1,2, Cintia W Rivero3,4

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A novel biocatalyst enhanced decitabine (DAC) biosynthesis, achieving high productivity. Immobilization techniques improved its stability and reusability, enabling a cost-effective and green production method.

Keywords:
BentoniteBiocatalysisImmobilizationNucleoside 2′-deoxyribosyltransferase

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Area of Science:

  • Biocatalysis
  • Green Chemistry
  • Nanobiotechnology

Background:

  • Decitabine (DAC) is a crucial antitumoral agent.
  • Developing efficient and stable biocatalysts for DAC biosynthesis is essential.
  • Existing methods may lack stability and cost-effectiveness.

Purpose of the Study:

  • To develop a highly productive and stable biocatalyst for decitabine (DAC) biosynthesis.
  • To improve the storage, reusability, and operational stability of the biocatalyst.
  • To optimize reaction conditions and scale-up the bioprocess for cost-effective and environmentally friendly DAC production.

Main Methods:

  • A novel IDA-LaNDT derivative was synthesized.
  • Ionic absorption and gel entrapment techniques were combined with bentonite incorporation for biocatalyst immobilization.
  • Reaction conditions, including 5-azacytidine solubility, were optimized using dimethyl sulfoxide.
  • The scale-up potential of the nanostabilized biocatalyst was evaluated.

Main Results:

  • The novel biocatalyst achieved high productivity in decitabine (DAC) biosynthesis.
  • Immobilization significantly enhanced storage stability (≥18 months), reusability (400 h), and thermal/solvent stability.
  • Optimized conditions and scale-up yielded 221 mg/L·h of DAC.
  • Green parameters confirmed a smooth, cheap, and environmentally friendly DAC biosynthesis methodology.

Conclusions:

  • The nanostabilized biocatalyst offers superior stability and reusability for decitabine (DAC) production.
  • This methodology represents a significant advancement in the green and cost-effective biosynthesis of decitabine.
  • The developed process is suitable for industrial scale-up, providing a sustainable route to an important antitumoral agent.