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Updated: Dec 7, 2025

Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
New Insight of Circular RNAs in Human Musculoskeletal Diseases
Yuzhe Liu1,2, Xinming Gu3, He Liu1,2
1Department of Orthopaedics of the Second Hospital, Jilin University, Changchun, China.
Abstract:
Circular RNAs (circRNAs), a novel group of noncoding RNAs, are present in most eukaryotic cells. Different from messenger RNAs, circRNAs have a covalently closed single-stranded stable structure and often act in cell type and tissue-specific manners, indicating that they can be used as biomarkers. With the advance of high-throughput RNA sequencing technology and bioinformatics, a large number of circRNAs have been identified in association with musculoskeletal diseases, but the functions of most circRNAs have not been clarified. circRNAs regulate biological processes by adsorbing microRNA as "sponges," binding to proteins, acting as transcriptional regulators, and participating in translation of proteins. In this study, we discuss the latest understanding of biogenesis and gene regulatory mechanisms of circRNAs with special emphasis on new targets for musculoskeletal disease diagnosis and clinical treatment.
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