Related Experiment Video
Updated: Dec 7, 2025

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis
Published on: December 8, 2023
Optimization of Method for Pesticide Detection in Honey by Using Liquid and Gas Chromatography Coupled with Mass
Mariana O Almeida1, Silvia Catarina S Oloris1, Vanessa Heloisa F Faria1
1Instituto Otávio Magalhães, Fundação Ezequiel Dias (Funed), Rua Conde Pereira Carneiro 80, Belo Horizonte 30510-010, MG, Brazil.
Abstract:
This study aimed to optimize and validate a multi-residue method for identifying and quantifying pesticides in honey by using both gas and liquid chromatographic separation followed by mass spectrometric detection. The proposed method was validated to detect 168 compounds, 127 of them by LC-MS/MS (liquid chromatography tandem mass spectrometric detection) and 41 by GC-MS/MS (gas chromatography tandem mass spectrometric detection). The limit of detection (LOD) and limit of quantification (LOQ) values for the analytes determined by LC-MS/MS were 0.0001-0.0004 mg/kg and 0.0002-0.0008 mg/kg, respectively. For GC-MS/MS analyses, the LOD and LOQ values were 0.001-0.004 mg/kg and 0.002-0.008 mg/kg. In total, 33 samples of commercial honey produced by apiaries in six Brazilian states were analyzed with the validated method. Residual amounts of 15 analytes were detected in 31 samples (93.9%). The method described in the present study was able to detect an extensive and broad range of pesticides with very high sensitivity.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-II
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

